Harpreet Vander
Panjab University, India
Title: Reproductive failure: An aftermath of vaginal colonization with sperm impairing microorganism in mouse model
Biography
Biography: Harpreet Vander
Abstract
Infertility in females as a consequence of asymptomatic microbial colonization of genital tract is under-acknowledged. A number of microorganisms have been known to elicit multiple deteriorative effects on sperm parameters in vitro, but their impact on fertility under in vivo conditions is still being argued. Earlier in our laboratory, infertility as a result of vaginal colonization with sperm impairing micro-organisms viz. Staphylococcus aureus, Escherichia coli, Serratia marcescens and Candida albicans has been observed. Therefore, the present study was designed to evaluate the impact of other uropathogens viz. sperm immobilizing Pseudomonas aeruginosa and non spermagglutinating/immobilizing Proteus mirabilis and Enterococcus faecalis on fertility outcome. The doses of 104, 106, 108 cfu of P. aeruginosa/P. mirabilis/E. faecalis was administered intra-vaginally into female Balb/c mice for 10 consecutive days followed by mating with proven breeder male on day 12. The results showed that female mice were rendered infertile in the group receiving P. aeruginosa. In contrast the group receiving non spermagglutinating/immobilizing strains showed abdominal distension, string of pearls and finally delivered pups at the end of gestation period. Further, no histopathological changes were observed in reproductive organs viz. ovary, uterus and vagina of mice in all the groups. Moreover, there were no significant changes in the malondialdehyde levels of vaginal tissue homogenates of all the groups as compared to control. In conclusion, female reproductive tract may be occasionally inhabited by uropathogens without producing any evident symptoms and this colonization with sperm impairing microbes can substantially contribute to adverse fertility outcomes.